Analytical Data
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Gene name
IL4
- Application
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Alternative Names
IL4;Interleukin-4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05112
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Expression Region
25-153aa
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AA Sequence
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAAT VLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIMREKYSKCSS
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Molecular Weight
15 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-4 (IL-4) is a crucial cytokine primarily produced by T-helper 2 (Th2) cells, playing a pivotal role in immune regulation and inflammation. Its involvement in the differentiation of B cells, the promotion of antibody production, and the enhancement of macrophage activation underscores its significance in various immune responses. Research on IL-4 recombination proteins has gained momentum due to their potential therapeutic applications in treating allergic diseases, autoimmune disorders, and even malignancies. The recombinant form of IL-4 allows for enhanced studies of its biochemical properties, functional assays, and interactions with specific receptors, providing insights into its mechanisms of action. Moreover, the production of IL-4 as a recombinant protein enables researchers to explore its role in modulating immune responses, particularly in the context of Th2-driven conditions such as asthma and atopic dermatitis. Recent advancements in recombinant DNA technology have facilitated the large-scale production of IL-4, enabling comprehensive studies on its pharmacokinetics, efficacy, and safety profiles. As the understanding of IL-4’s multifunctional nature evolves, research continues to uncover novel pathways and therapeutic strategies, highlighting the importance of IL-4 in both basic and clinical immunology.
