Analytical Data
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基因名
IL4
- Application
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别名
IL4;Interleukin-4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P05112
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表达区间
25-153aa
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氨基酸序列
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAAT VLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIMREKYSKCSS
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分子量
15 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-4 (IL-4) is a crucial cytokine primarily produced by T-helper 2 (Th2) cells, playing a pivotal role in immune regulation and inflammation. Its involvement in the differentiation of B cells, the promotion of antibody production, and the enhancement of macrophage activation underscores its significance in various immune responses. Research on IL-4 recombination proteins has gained momentum due to their potential therapeutic applications in treating allergic diseases, autoimmune disorders, and even malignancies. The recombinant form of IL-4 allows for enhanced studies of its biochemical properties, functional assays, and interactions with specific receptors, providing insights into its mechanisms of action. Moreover, the production of IL-4 as a recombinant protein enables researchers to explore its role in modulating immune responses, particularly in the context of Th2-driven conditions such as asthma and atopic dermatitis. Recent advancements in recombinant DNA technology have facilitated the large-scale production of IL-4, enabling comprehensive studies on its pharmacokinetics, efficacy, and safety profiles. As the understanding of IL-4’s multifunctional nature evolves, research continues to uncover novel pathways and therapeutic strategies, highlighting the importance of IL-4 in both basic and clinical immunology.












