Analytical Data
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Gene name
MANF
- Application
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Alternative Names
MGC142148; MGC142150; Protein ARMET
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55145
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Expression Region
25-182aa
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AA Sequence
LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
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Molecular Weight
18.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor) is a neurotrophic factor that plays a crucial role in neuroprotection and the survival of neurons under stress conditions. Discovered in the mid-2000s, MANF is primarily expressed in the endoplasmic reticulum of astrocytes and has been shown to have significant implications in various neurodegenerative diseases, including Parkinson's disease, Alzheimer's disease, and multiple sclerosis. Research has highlighted its potential to promote cell survival, support neuronal differentiation, and modulate inflammation. Moreover, MANF has been associated with the inhibition of apoptosis and the enhancement of the adaptive stress response, which is critical in maintaining neuronal health. The recombinant production of MANF has garnered attention due to its therapeutic potential, leading to investigations into its role in enhancing neuronal resilience and repair mechanisms. Studies involving recombinant MANF have explored its effects on various neuronal models, revealing its promise as a neuroprotective agent. As such, ongoing research aims to elucidate the precise mechanisms by which MANF exerts its beneficial effects, assess its therapeutic applications in clinical settings, and explore its potential as a target for drug development in treating neurodegenerative disorders. Overall, the understanding of MANF and its recombinant forms represents a significant venture towards developing novel neuroprotective strategies aimed at combating debilitating neurodegenerative diseases.
