Analytical Data
-
Gene name
P4Ha1
- Application
-
Alternative Names
P4Ha1；P4HA；Prolyl 4-hydroxylase subunit alpha-1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P13674
-
Expression Region
1-534aa
-
AA Sequence
MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEE DKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELEN LVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISK GNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEIS TIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEY IMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKM TPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE IVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMR IQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRI ATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRH AACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE
-
Molecular Weight
85 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
P4Ha1, or prolyl 4-hydroxylase alpha subunit 1, is a crucial enzyme involved in the post-translational modification of collagen, a major structural protein in the extracellular matrix. The hydroxylation of proline residues by P4Ha1 is essential for the stability and function of collagen, influencing collagen synthesis and assembly. Dysregulation of P4Ha1 has been implicated in various pathological conditions, including fibrosis, cancer, and cardiovascular diseases, making it a significant target for therapeutic intervention. Recent research has focused on the role of P4Ha1 not only in collagen metabolism but also in cellular signaling pathways and hypoxia responses, as it is sensitive to oxygen levels. Understanding the structure-function relationships of P4Ha1 and its interactions with other proteins can provide insights into its regulatory mechanisms and potential to serve as a biomarker for disease progression. Additionally, advancements in recombinant protein technology have enabled the production of P4Ha1 in larger quantities, facilitating experimental studies and potential drug development. This body of research underscores the importance of P4Ha1 in both basic biology and clinical applications, highlighting its role as a significant player in collagen-related diseases and tissue remodeling processes.
