Analytical Data
-
Gene name
GJb1
- Application
-
Alternative Names
GJb1；CX32；Gap junction beta-1 Protein
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P08034
-
Expression Region
1-283aa
-
AA Sequence
MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDQFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCDVYPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC
-
Molecular Weight
32 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GJB1, also known as Gap Junction Protein Beta 1, is a crucial component of gap junctions, which facilitate direct intercellular communication through the exchange of ions and small molecules. Mutations in the GJB1 gene are associated with various neurological disorders, particularly Charcot-Marie-Tooth disease type 1X (CMT1X), a hereditary neuropathy that significantly affects motor and sensory functions. The study of GJB1 recombinant proteins has gained prominence due to its potential for elucidating the mechanisms underlying these mutations and their phenotypic manifestations. Additionally, researching GJB1 can provide insights into the physiological roles of gap junctions in cellular communication, which is vital for maintaining tissue homeostasis and function. The recombinant expression of GJB1 allows for the production of functional proteins that can be used in both in vitro and in vivo models, facilitating the exploration of their physiological and pathological roles. Understanding the structure-function relationship of GJB1 is fundamental to developing therapeutic strategies for conditions arising from its dysfunction. Thus, research on GJB1 recombinant proteins is critical not only for advancing genetic and molecular understanding of related diseases but also for exploring innovative therapeutic approaches to mitigate their effects.
