Analytical Data
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基因名
IMP-3
- Application
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别名
IGF2 mRNA-binding protein 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00425
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表达区间
1-579aa
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氨基酸序列
MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK
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分子量
69.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IMP-3, also known as IGF2BP3 (Insulin-like Growth Factor 2 mRNA Binding Protein 3), is an RNA-binding protein that plays a critical role in cellular processes such as mRNA stability, translation, and regulation of gene expression. It is known to be overexpressed in various malignancies, including cancers of the lung, breast, and gastrointestinal tract, making it of significant interest in cancer research. The study of IMP-3 and its recombinant protein has been driven by its potential as a biomarker for cancer diagnosis and prognosis as well as a therapeutic target. Research indicates that IMP-3 contributes to tumor progression by stabilizing oncogenic mRNAs and promoting cancer cell survival. Additionally, its interaction with other regulatory molecules can influence pathways crucial for cell growth and differentiation. Understanding the structure and function of the IMP-3 recombinant protein may aid in elucidating its precise mechanisms in oncogenesis and tumor microenvironment interactions. Due to its unique expression profile in cancerous tissues as compared to normal tissues, IMP-3 is being explored not only for its role in cancer biology but also for developing targeted therapies and novel diagnostic tools that could improve patient outcomes. The ongoing research aims to characterize the functional domains of the IMP-3 protein, its RNA targets, and downstream signaling pathways, thereby providing a comprehensive understanding of its involvement in cancer biology and potential clinical applications.
