Analytical Data
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Gene name
Atp1a3
- Application
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Alternative Names
Atp1a3；Sodium/potassium-transporting ATPase subunit alpha-3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13637
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Expression Region
1-1013aa
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AA Sequence
MGDKKDDKDSPKKNKGKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCV QGLTHSKAQEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAIL CFLAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQEAKSSKIMES FKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHG CKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVA TGDRTVMGRIATLASGLEVGKTPIAIEIEHFIQLITGVAVFLGVSFFILS LILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKN LEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTS FDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCI ELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGA PERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYL PEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAG IKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDA KACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGA IVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVT GVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCI DLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLISMAYGQIGMI QALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQ RKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFE ETALAAFLSYCPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILR RNPGGWVEKETYY
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Molecular Weight
138 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATP1A3 is a critical component of the Na+/K+ ATPase enzyme, which is essential for maintaining cellular ion homeostasis by transporting sodium and potassium ions across the cell membrane. Mutations in the ATP1A3 gene have been implicated in various neurodevelopmental disorders, such as Alternating Hemiplegia of Childhood (AHC) and ATP1A3-related epilepsy. These conditions highlight the importance of understanding ATP1A3 function and regulation in neuronal activity and overall brain health. Due to its role in ion transport and cellular signaling, ATP1A3 reconstituted protein studies aim to dissect the molecular mechanisms underlying its activity, interaction with other proteins, and response to pharmacological agents. The production of recombinant ATP1A3 provides an invaluable tool for biophysical and biochemical analyses, enabling researchers to investigate its structure-function relationships in isolation from the complexities of cellular environments. Furthermore, understanding how specific mutations affect ATP1A3's activity can offer insights into disease mechanisms, potentially guiding the development of targeted therapies for related disorders. Overall, research on ATP1A3 reconstituted proteins is crucial for elucidating the pathways impacted by ion transport dysregulation and advancing our understanding of associated clinical conditions.
