Analytical Data
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Gene name
PPO2
- Application
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Alternative Names
PPO2；ADPRT2；ADPRTL2；Poly [ADP-ribose] polymerase 2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O42713
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Expression Region
1-378aa
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AA Sequence
MSLIATVGPTGGVKNRLNIVDFVKNEKFFTLYVRSLELLQAKEQHDYSSFFQLAGIHGLPFTEWAKERPSMNLYKAGYCTHGQVLFPTWHRTYLSVLEQILQGAAIEVAKKFTSNQTDWVQAAQDLRQPYWDWGFELMPPDEVIKNEEVNITNYDGKKISVKNPILRYHFHPIDPSFKPYGDFATWRTTVRNPDRNRREDIPGLIKKMRLEEGQIREKTYNMLKFNDAWERFSNHGISDDQHANSLESVHDDIHVMVGYGKIEGHMDHPFFAAFDPIFWLHHTNVDRLLSLWKAINPDVWVTSGRNRDGTMGIAPNAQINSETPLEPFYQSGDKVWTSASLADTARLGYSYPDFDKLVGGTKELIRDAIDDLIDERYG
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Molecular Weight
51.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PPO2, or Polyphenol Oxidase 2, is a significant enzyme involved in the browning process of fruits and vegetables, as well as in various biochemical pathways related to plant defense mechanisms. The research background for PPO2 recombinant protein is grounded in its crucial role in catalyzing the oxidation of phenolic compounds, leading to the production of brown pigments, which can affect the aesthetic and nutritional quality of food products. Understanding the structure and function of PPO2 is essential for several applications, including food preservation, agricultural biotechnology, and the development of strategies to mitigate enzymatic browning in crops. Recent advances in molecular biology and protein engineering have enabled scientists to produce PPO2 as a recombinant protein, facilitating detailed studies of its enzymatic characteristics, kinetics, and potential inhibitors. Such research not only strives to elucidate the enzyme's mechanisms but also explores its utility in industrial applications, including developing natural food preservatives and enhancing the shelf life of perishable goods. By harnessing the properties of recombinant PPO2, researchers aim to improve food quality and safety while providing insights into enzyme regulation within plant systems, thus contributing to agricultural innovation and sustainability.
