Analytical Data
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基因名
CD73
- Application
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别名
CD73；NT5；NTE；5'-nucleotidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P21589
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表达区间
27-547aa
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氨基酸序列
WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHT DEMFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI K
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分子量
59 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD73, also known as ecto-5'-nucleotidase, is an important enzyme involved in purine metabolism and plays a pivotal role in the immune system by converting extracellular ATP into adenosine. This process is critical as adenosine functions as a signaling molecule that can modulate immune responses, promoting tolerance and suppressing inflammation. The expression of CD73 is upregulated in various pathological conditions, including cancer, where it can facilitate tumor progression by creating an immunosuppressive microenvironment. This has garnered significant interest in understanding its role in cancer immunity and the potential of CD73 as a therapeutic target. Research on recombinant CD73 protein is essential for elucidating its structure, function, and mechanisms of action. By generating and characterizing recombinant CD73, scientists can study its enzymatic activity, binding properties, and interactions with other molecules. This knowledge can facilitate the development of CD73 inhibitors or agonists that could be applied in clinical settings, particularly in oncology and autoimmune diseases. Additionally, recombinant CD73 can be used as a biomarker for disease progression or treatment response, further emphasizing its importance in biomedical research. Overall, the study of recombinant CD73 protein is crucial for advancing our understanding of its biological roles and therapeutic potential in modulating immune responses.
