Analytical Data
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基因名
GTF2B
- Application
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别名
GTF2B;TF2B;TFIIB;Transcription initiation factor IIB
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00403
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表达区间
1-316aa
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氨基酸序列
MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL
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分子量
50.8kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2B (General Transcription Factor II B) is a crucial protein involved in the initiation of transcription by RNA polymerase II, playing a pivotal role in the assembly of the transcription machinery and regulation of gene expression. The interest in GTF2B recombinant protein studies stems from its significance in the transcriptional regulation process, where it acts as a cofactor that bridges the RNA polymerase II and the transcriptional complex. Dysfunctional GTF2B has been linked to various diseases, including certain cancers and developmental disorders. Recent research efforts have focused on the production of high-purity recombinant GTF2B, allowing for detailed biochemical and structural analysis. This enables scientists to investigate its interactions with other transcription factors, characterize its functional domains, and understand its regulatory mechanisms. Such studies are crucial for deciphering the complexities of gene expression and could pave the way for targeted therapeutic strategies aimed at diseases related to transcriptional misregulation. Furthermore, understanding GTF2B's role in the context of other transcription factors may reveal insights into its contribution to epigenetic modifications and long-range gene regulation. Overall, the characterization of GTF2B as a recombinant protein provides a foundational platform for advancing our knowledge of transcription mechanisms and their implications in health and disease.
