Analytical Data
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Gene name
mutL
- Application
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Alternative Names
mutL；DNA mismatch repair Protein Mlh3
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P23367
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Expression Region
1-615aa
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AA Sequence
MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDIDIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLERDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALAELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLMSEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE
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Molecular Weight
71.9kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MutL is a critical protein involved in the DNA mismatch repair (MMR) system, which plays a pivotal role in maintaining genomic stability by correcting errors that occur during DNA replication. This protein is part of a larger complex that identifies and repairs mispaired nucleotides, thereby preventing mutations that could lead to cancer and other genetic disorders. MutL functions by interacting with other MMR proteins, such as MutS, which recognizes the mismatch, and MutH, which introduces a cleavage near the error, enabling the removal and correct replacement of the erroneous nucleotides. Research into MutL has expanded our understanding of its structure, function, and the intricate mechanisms by which it coordinates the repair process. Studies have revealed its dual role in not only facilitating mismatch repair but also in regulating recombination and cellular responses to DNA damage. Furthermore, mutations in the gene encoding MutL are associated with hereditary non-polyposis colorectal cancer (HNPCC), highlighting the clinical significance of elucidating its function and mechanisms. Advances in techniques such as X-ray crystallography and cryo-electron microscopy have provided deeper insights into the conformational changes and dynamics of MutL during the repair process. Consequently, ongoing research into MutL not only enhances our understanding of the fundamental processes governing DNA integrity but also holds potential for developing therapeutic strategies to target cancers associated with MMR deficiencies.
