Analytical Data
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Gene name
GPX3
- Application
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Alternative Names
GPX3;GPXP;Glutathione peroxidase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P22352
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Expression Region
21-226aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MQSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNV ASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGD VNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKM DILSYMRRQA ALGVKRK
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Molecular Weight
26 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GPX3, or glutathione peroxidase 3, is a selenium-dependent antioxidant enzyme that plays a crucial role in protecting cells from oxidative stress by catalyzing the reduction of hydrogen peroxide and lipid peroxides. It is primarily expressed in plasma and various tissues, indicating its importance in maintaining cellular redox homeostasis. Research into GPX3 has gained traction due to its potential implications in various diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders, where oxidative stress is a contributing factor. Furthermore, studies have identified GPX3's regulatory role in the immune response and its association with inflammatory processes. Advances in recombinant protein technology have facilitated the production and purification of GPX3, enabling researchers to study its structure-function relationships, enzyme kinetics, and interactions with other biomolecules. Investigating GPX3 through recombinant protein approaches not only enhances our understanding of its physiological functions but also opens avenues for therapeutic applications, such as the development of targeted antioxidants and novel treatments for oxidative stress-related diseases. As the demand for effective strategies to combat oxidative damage continues to rise, GPX3 stands out as a promising candidate for further exploration in both basic and applied research realms.
