Analytical Data
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基因名
GOT2
- Application
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别名
GOT2;KYAT4;Aspartate aminotransferase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00505
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表达区间
30-430aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSSSWWTHVEMGPPDPILGVTEAFKRDTN SKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFC KASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVF LPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSV LLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDK DAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRT QLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDG RISVAGVTSSNVGYLAHAIHQVTK
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分子量
47 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GOT2 (Glutamate Oxidoreductase 2) is a vital enzyme involved in the mitochondrial pathway of amino acid metabolism, primarily responsible for the reversible conversion of L-aspartate and α-ketoglutarate to L-glutamate and oxaloacetate. This enzyme plays a crucial role in the cellular metabolism and energy production within mitochondria, impacting various physiological processes such as neurotransmitter regulation and nitrogen metabolism. The significance of GOT2 has been highlighted in the context of various diseases, including neurodegenerative disorders and cancers, where its expression levels and activity can be altered. Researchers are keenly investigating the potential of GOT2 as a therapeutic target and biomarker due to its involvement in metabolic pathways that are often dysregulated in pathological conditions. The development of recombinant GOT2 proteins allows for in-depth studies of its biochemical properties, interaction with inhibitors, and role in metabolic flux, enabling scientists to elucidate its function and regulatory mechanisms in health and disease. Such studies hold promise for advancing our understanding of mitochondrial dysfunction and developing novel therapeutic strategies aimed at restoring normal metabolic processes. Overall, the ongoing research into recombinant GOT2 not only expands our basic understanding of mitochondrial metabolism but also paves the way for innovative approaches to target metabolic diseases effectively.
