Analytical Data
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Gene name
PDHA1
- Application
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Alternative Names
PDHA1；PHE1A；Pyruvate dehydrogenase E1 component subunit alpha. somatic form. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08559
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Expression Region
31-390aa
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AA Sequence
ANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
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Molecular Weight
71.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDHA1 (Pyruvate Dehydrogenase E1 Alpha) is a crucial enzyme in the mitochondrial pyruvate dehydrogenase complex, which plays a vital role in cellular metabolism by catalyzing the conversion of pyruvate into acetyl-CoA. This metabolic pathway is essential for the aerobic oxidation of carbohydrates and fatty acids, making PDHA1 integral to energy production in cells. Mutations in the PDHA1 gene can lead to pyruvate dehydrogenase deficiency, a condition associated with neurological disorders and metabolic dysregulation. Research into PDHA1 recombinant protein is significant for understanding its structure-function relationship, therapeutic targeting, and potential for gene therapy. By producing recombinant PDHA1, scientists can explore the enzyme's kinetic properties, stability, and interactions with other metabolic pathways. Additionally, the recombinant protein serves as a valuable tool for screening potential inhibitors or activators, which can advance our understanding of metabolic diseases and aid in the development of metabolic modulators. Furthermore, studying PDHA1 provides insights into mitochondrial function and its implications in various disorders, including cancer, where altered energy metabolism is frequently observed. This research not only enhances our knowledge of basic metabolic processes but also opens avenues for novel therapeutic strategies in metabolic and neurodegenerative diseases linked to PDHA1 dysfunction.
