Analytical Data
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Gene name
GOT1
- Application
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Alternative Names
GOLT1A;GOT1B;Vesicle transport Protein GOT1A
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Species
Mouse
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05201
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Expression Region
2-413aa
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AA Sequence
APPSVFAQV PQAPPVLVFK LTADFRDDPD PRKVNLGVGA YRTDESQPWV LPVVRKVEQK IANDNSLNHE YLPILGLAEF RSCASRLVLG DNSLAIRENR VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPIYVSSPT WENHNAVFSA AGFKDIRPYC YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD PTPEQWKQIA AVMQRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVGKESDS VLRVLSQMEK IVRITWSNPP AQGARIVAAT LSDPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINM CGLTTKNLDY VATSIHEAVT KIQ
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GOT1, or glutamate-oxaloacetate transaminase 1, is a pivotal enzyme in amino acid metabolism, playing a crucial role in the transamination process that regulates the balance of amino acids and the overall metabolism of nitrogen in cells. This enzyme is primarily localized in the mitochondria and is involved in the interconversion of aspartate and oxaloacetate, a reaction that links the urea cycle and the citric acid cycle. Research on GOT1 recombinant protein has gained momentum due to its potential implications in various metabolic disorders, neurodegenerative diseases, and cancer. By understanding the structure-function relationship of GOT1, scientists aim to elucidate its roles in cellular metabolism and explore its potential as a therapeutic target. Additionally, recombinant protein studies facilitate the investigation of enzyme kinetics, inhibition, and substrate specificity, which are critical for drug design and development. The production of GOT1 in a heterologous system allows for extensive biochemical characterization and opens pathways for developing diagnostics and treatments for diseases associated with disrupted amino acid metabolism. Consequently, the study of GOT1 recombinant protein not only enhances our fundamental knowledge of metabolic processes but also holds significant promise for advancing medical research and therapeutic innovations.
