Analytical Data
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Gene name
NFKB3
- Application
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Alternative Names
NFKB3；NFKB3；Transcription factor p65
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q04206
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Expression Region
1-551aa
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AA Sequence
MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
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Molecular Weight
60.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NFKB3, also known as nuclear factor kappa-light-chain-enhancer of activated B cells, is a pivotal transcription factor involved in regulating immune response, inflammation, and cell survival. The NFKB family consists of several members, with NFKB3 (also referred to as p50 or NF-kB1) playing a significant role in various biological processes. Research has highlighted its importance in the pathogenesis of numerous diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions. Under normal physiological conditions, NFKB3 is retained in the cytoplasm in an inactive form bound to IκB inhibitors. Upon stimulation by pro-inflammatory cytokines or other stress signals, IκB proteins are phosphorylated, leading to the subsequent release and nuclear translocation of NFKB3, where it binds to specific DNA sequences to activate target genes. The dysregulation of NFKB3 has been implicated in tumorigenesis and metastasis, making it an attractive target for therapeutic intervention. Recent advances in recombinant protein technology have facilitated the production of NFKB3 in a purer and more functional form, enabling detailed studies on its structure, function, and interaction with other proteins. Understanding these interactions is critical for developing targeted therapies that could modulate NFKB3 activity, offering potential avenues for innovative treatments in diseases characterized by persistent inflammation and immune dysfunction. As research progresses, insights gained from NFKB3 recombinant protein studies may lead to novel strategies to manage and treat various diseases by precisely regulating this crucial signaling pathway.
