Analytical Data
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基因名
fabH
- Application
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别名
fabH；FABP11；MDGI；Fatty acid-binding Protein. heart
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5HHA2
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表达区间
1-313aa
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氨基酸序列
MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHWADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGKVASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDGAGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDASTRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPLSIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK
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分子量
41.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FabH, or β-ketoacyl-acyl carrier protein synthase, is a critical enzyme in the fatty acid biosynthesis pathway, predominantly found in bacteria and some plants. It catalyzes the initial step of the type II fatty acid synthesis, which is essential for the production of membrane lipids and signal molecules in various organisms. The significance of fabH lies in its potential as a target for antibiotic development, particularly against pathogenic bacteria that possess a type II fatty acid biosynthesis system, distinguishing them from mammalian cells that utilize a type I pathway. Research on the recombinant expression of FabH allows for detailed study of its enzymatic mechanisms, substrate specificity, and structural characteristics through techniques such as crystallography and NMR spectroscopy. By producing recombinant FabH, scientists can investigate its functionalities more comprehensively, paving the way for the design of inhibitors that can disrupt bacterial growth and proliferation. Furthermore, understanding FabH's role in fatty acid metabolism contributes to broader applications in biotechnology, including the engineering of microbial strains for the production of biofuels and bioplastics. The exploration of fabH thus represents a promising avenue in both fundamental research and practical applications, highlighting its importance in microbial physiology and the quest for new antimicrobial strategies.
