Analytical Data
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Gene name
fabH
- Application
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Alternative Names
fabH；FABP11；MDGI；Fatty acid-binding Protein. heart
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5HHA2
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Expression Region
1-313aa
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AA Sequence
MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHWADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGKVASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDGAGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDASTRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPLSIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK
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Molecular Weight
41.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FabH, or β-ketoacyl-acyl carrier protein synthase, is a critical enzyme in the fatty acid biosynthesis pathway, predominantly found in bacteria and some plants. It catalyzes the initial step of the type II fatty acid synthesis, which is essential for the production of membrane lipids and signal molecules in various organisms. The significance of fabH lies in its potential as a target for antibiotic development, particularly against pathogenic bacteria that possess a type II fatty acid biosynthesis system, distinguishing them from mammalian cells that utilize a type I pathway. Research on the recombinant expression of FabH allows for detailed study of its enzymatic mechanisms, substrate specificity, and structural characteristics through techniques such as crystallography and NMR spectroscopy. By producing recombinant FabH, scientists can investigate its functionalities more comprehensively, paving the way for the design of inhibitors that can disrupt bacterial growth and proliferation. Furthermore, understanding FabH's role in fatty acid metabolism contributes to broader applications in biotechnology, including the engineering of microbial strains for the production of biofuels and bioplastics. The exploration of fabH thus represents a promising avenue in both fundamental research and practical applications, highlighting its importance in microbial physiology and the quest for new antimicrobial strategies.
