Analytical Data
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基因名
GID8
- Application
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别名
GID8；C20orf11；TWA1;Glucose-induced degradation Protein 8 homolog
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NWU2
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表达区间
1-228aa
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氨基酸序列
MSYAEKPDEI TKDEWMEKLN NLHVQRADMN RLIMNYLVTE GFKEAAEKFR MESGIEPSVD LETLDERIKI REMILKGQIQ EAIALINSLH PELLDTNRYL YFHLQQQHLI ELIRQRETEA ALEFAQTQLA EQGEESRECL TEMERTLALL AFDSPEESPF GDLLHTMQRQ KVWSEVNQAV LDYENRESTP KLAKLLKLLL WAQNELDQKK VKYPKMTDLS KGVIEEPK
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GID8, a member of the GID complex, plays a crucial role in the ubiquitin-proteasome pathway, specifically in the targeted degradation of proteins. It has garnered significant attention due to its involvement in various cellular processes, including cell cycle regulation and response to environmental stress. Studies have shown that GID8 functions as an E3 ubiquitin ligase, facilitating the attachment of ubiquitin moieties to substrate proteins, thus marking them for proteasomal degradation. This mechanism is essential for maintaining cellular homeostasis and regulating protein turnover, which is vital for proper cell function and adaptation to stressors. Moreover, dysregulation of GID8 is implicated in several diseases, including cancer, where altered protein degradation pathways may contribute to uncontrolled cell proliferation. Current research focuses on elucidating the structural and functional properties of GID8, understanding its interactions with various substrates, and exploring its potential as a therapeutic target. By investigating the molecular mechanisms by which GID8 operates, scientists aim to develop strategies to modulate its activity, offering new avenues for treating diseases linked to its dysfunction. This research has broader implications for understanding the ubiquitin-proteasome system's role in cellular regulation and targeting protein degradation as a therapeutic strategy.
