Analytical Data
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基因名
GCLM
- Application
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别名
GCLM；GLCLR;Glutamate--cysteine ligase regulatory subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48507
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表达区间
1-274aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSHMGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS
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分子量
33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GCLM, or Glutamate-Cysteine Ligase Modifier Subunit, plays a critical role in the regulation of glutathione synthesis, which is essential for cellular antioxidant defense and detoxification processes. The enzyme complex, consisting of a catalytic subunit (GCLC) and a modifier subunit (GCLM), is pivotal in maintaining cellular redox balance and protecting against oxidative stress. Research into GCLM recombinant proteins has gained momentum due to its implications in various pathological conditions, including neurodegenerative diseases, cancer, and metabolic disorders. Understanding the function and regulation of GCLM can provide insights into therapeutic strategies aimed at enhancing antioxidant defenses in disease states. Recombinant GCLM offers a tool for detailed biochemical studies, allowing researchers to unravel the mechanistic underpinnings of glutathione biosynthesis and its impact on cellular health. Enhanced production of GCLM through recombinant techniques has the potential to facilitate new therapeutic interventions that target oxidative stress-related diseases, emphasizing the importance of this protein in biomedical research.
