Analytical Data
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基因名
MYLK4
- Application
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别名
MYLK4；SGK085；Myosin light chain kinase family member 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86YV6
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表达区间
1-388aa
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氨基酸序列
MLKVKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGDQDSRSR HNEAKEVWSNADLTERMPVKSKRTSALAVDIPAPPAPFDHRIVTAKQGAV NSFYTVSKTEILGGGRFGQVHKCEETATGLKLAAKIIKTRGMKDKEEVKN EISVMNQLDHANLIQLYDAFESKNDIVLVMEYVDGGELFDRIIDESYNLT ELDTILFMKQICEGIRHMHQMYILHLDLKPENILCVNRDAKQIKIIDFGL ARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFPTDMWSVGVIAYMLLSGL SPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRI SASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK
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分子量
70 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MYLK4 (Myosin Light Chain Kinase 4) is a member of the myosin light chain kinase family, which plays a critical role in cellular processes such as muscle contraction, cell motility, and signal transduction. As a serine/threonine kinase, MYLK4 specifically phosphorylates the regulatory light chain of myosin, thereby influencing actin-myosin interactions and cellular mechanics. Research into MYLK4 has gained momentum due to its potential implications in various physiological and pathological conditions, including cardiac function, smooth muscle contraction, and certain cancers. It has been observed that alterations in MYLK4 expression and activity can significantly impact cell behavior, making it a target of interest for therapeutic strategies. Understanding the role of MYLK4 at a molecular level can shed light on its contributions to muscle-related diseases and provide insights into therapeutic interventions for cancer and vascular disorders. The production and study of recombinant MYLK4 proteins allow researchers to investigate its biochemical properties, interactions with other molecules, and regulatory mechanisms. Such studies are essential for elucidating the functional role of MYLK4 in health and disease, potentially leading to new avenues for drug development and targeted therapies that exploit the kinase's specific activity.
