Analytical Data
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Gene name
GTF2H2
- Application
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Alternative Names
Basic transcription factor 2 44 kDa subunit; BTF2; BTF2 p44; BTF2-p44; BTF2P44; General transcription factor IIH; General transcription factor IIH polypeptide 2; General transcription factor IIH subunit 2; General transcription factor IIH; polypeptide 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13888
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Expression Region
1-395aa
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AA Sequence
MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS IAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK AAKIRVSVIG LSAEVRVCTV LARETGGTYH VILDESHYKE LLTHHVSPPP ASSSSECSLI RMGFPQHTIA SLSDQDAKPS FSMAHLDGNT EPGLTLGGYF CPQCRAKYCE LPVECKICGL TLVSAPHLAR SYHHLFPLDA FQEIPLEEYN GERFCYGCQG ELKDQHVYVC AVCQNVFCVD CDVFVHDSLH CCPGCIHKIP APSGV
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Molecular Weight
44.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2H2, a subunit of the general transcription factor IIH complex, plays a critical role in the process of transcription initiation and DNA repair. It is involved in the recruitment of RNA polymerase II and has essential functions in nucleotide excision repair (NER), a mechanism vital for maintaining genomic integrity. The study of GTF2H2 is significant due to its implications in various biological processes and diseases, including cancer, where defects in DNA repair mechanisms can lead to tumorigenesis. Researchers have focused on characterizing the protein's structure, functional domains, and interaction partners to elucidate its role in transcriptional regulation and DNA repair pathways. Additionally, recombinant GTF2H2 has become a key tool for investigating these processes in vitro, enabling better understanding of its contributions to transcriptional regulation and providing insights into potential therapeutic targets. Understanding GTF2H2's mechanisms may lead to novel strategies for enhancing gene expression and improving cellular responses to DNA damage, ultimately contributing to advances in cancer treatment and gene therapy.
