Analytical Data
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基因名
GTF2H2
- Application
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别名
Basic transcription factor 2 44 kDa subunit; BTF2; BTF2 p44; BTF2-p44; BTF2P44; General transcription factor IIH; General transcription factor IIH polypeptide 2; General transcription factor IIH subunit 2; General transcription factor IIH; polypeptide 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13888
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表达区间
1-395aa
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氨基酸序列
MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS IAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK AAKIRVSVIG LSAEVRVCTV LARETGGTYH VILDESHYKE LLTHHVSPPP ASSSSECSLI RMGFPQHTIA SLSDQDAKPS FSMAHLDGNT EPGLTLGGYF CPQCRAKYCE LPVECKICGL TLVSAPHLAR SYHHLFPLDA FQEIPLEEYN GERFCYGCQG ELKDQHVYVC AVCQNVFCVD CDVFVHDSLH CCPGCIHKIP APSGV
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分子量
44.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2H2, a subunit of the general transcription factor IIH complex, plays a critical role in the process of transcription initiation and DNA repair. It is involved in the recruitment of RNA polymerase II and has essential functions in nucleotide excision repair (NER), a mechanism vital for maintaining genomic integrity. The study of GTF2H2 is significant due to its implications in various biological processes and diseases, including cancer, where defects in DNA repair mechanisms can lead to tumorigenesis. Researchers have focused on characterizing the protein's structure, functional domains, and interaction partners to elucidate its role in transcriptional regulation and DNA repair pathways. Additionally, recombinant GTF2H2 has become a key tool for investigating these processes in vitro, enabling better understanding of its contributions to transcriptional regulation and providing insights into potential therapeutic targets. Understanding GTF2H2's mechanisms may lead to novel strategies for enhancing gene expression and improving cellular responses to DNA damage, ultimately contributing to advances in cancer treatment and gene therapy.
