Analytical Data
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Gene name
SIZ1
- Application
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Alternative Names
SIZ1；E3 SUMO-Protein ligase SIZ1
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q680Q4
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Expression Region
1-171aa
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AA Sequence
MDLEANCKEKLSYFRIKELKDVLTQLGLSKQGKKQELVDRILTLLSDEQAARLLSKKNTVAKEAVAKLVDDTYRKMQVSGASDLASKGQVSSDTSNLKVKGEPEDPFQPEIKVRCVCGNSLETDSMIQCEDPRCHVWQHVGCVILPDKPMDGNPPLPESFYCEICRLTRAD
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Molecular Weight
23.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SIZ1 (Suppressor of Igs1 1) is a SUMO E3 ligase that plays a crucial role in post-translational modifications through its involvement in the SUMOylation process, which regulates various cellular functions, including gene expression, DNA repair, and stress responses. The study of SIZ1 has gained significant attention due to its implications in plant biology, particularly in the model organism Arabidopsis thaliana, where it has been linked to various stress tolerance mechanisms, such as those against drought and high salinity. Understanding the function and regulation of SIZ1 may provide insights into the molecular pathways that plants employ to adapt to environmental stressors. Moreover, SIZ1's role in modulating protein interactions and stability positions it as a pivotal factor in the intricate networks governing cellular responses. Research into SIZ1 has the potential to contribute to the development of crops with enhanced resilience to abiotic stresses, making it a significant target for biotechnological applications. Overall, the investigation of SIZ1 and its associated pathways not only enriches our understanding of plant biology but also opens up new avenues for improving agricultural sustainability in the face of climate change.
