Analytical Data
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Gene name
KRT25
- Application
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Alternative Names
KRT25；KRT25A；Keratin. type I cytoskeletal 25
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z3Z0
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Expression Region
1-450aa
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AA Sequence
MSLRLSSASR RSCPRPTTGS LRLYGGGTSF GTGNSCGISG IGSGFSSAFG GSSSGGNTGG GNPCAGFTVN ERGLLSGNEK VTMQNLNDRL ASYLDSVHAL EEANADLEQK IKGWYEKFGP GSCRGLDHDY SRYFPIIDDL KNQIIASTTS NANAVLQIDN ARLTADDFRL KYENELALHQ SVEADVNGLR RVLDEITLCR TDLEIQYETL SEEMTYLKKN HKEEMQVLQC AAGGNVNVEM NAAPGVDLTV LLNNMRAEYE ALAEQNRRDA EAWFNEKSAS LQQQISEDVG ATTSARNELT EMKRTLQTLE IELQSLLATK HSLECSLTET ESNYCAQLAQ IQAQIGALEE QLHQVRTETE GQKLEYEQLL DIKLHLEKEI ETYCLLIGGD DGACKSGGYK SKDYGSGNVG SQVKDPAKAI VVKKVLEEVD QRSKILTTRL HSLEEKSQSN
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Molecular Weight
49.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KRT25, or keratin 25, is a member of the keratin family, which consists of fibrous structural proteins that play a crucial role in the integrity and resilience of epithelial cells. Research into KRT25 has emerged from the understanding that keratins are essential for maintaining cellular architecture and function in diverse tissues, particularly those undergoing constant mechanical stress, such as the skin and hair. Recently, studies have suggested that KRT25 may be involved in the development of certain skin disorders and hair abnormalities, making it a significant focus in dermatological research. The recombinant production of KRT25 protein allows scientists to investigate its biological properties and functional roles in vitro and in vivo. By utilizing recombinant techniques, researchers aim to elucidate the protein's structure, stability, and interaction with other keratin types, ultimately contributing to a better understanding of its role in skin health and disease. Moreover, elucidating the molecular mechanisms underlying KRT25's functions could pave the way for potential therapeutic applications in conditions linked to keratin dysfunction. In summary, the study of recombinant KRT25 protein is essential for unraveling the complex biology of keratins and advancing our knowledge of epithelial integrity, disease mechanisms, and potential treatment strategies.
