Analytical Data
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Gene name
FBXO4
- Application
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Alternative Names
FBXO4；FBX4；F-box only Protein 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKT5
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Expression Region
1-387aa
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AA Sequence
MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR
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Molecular Weight
48.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FBXO4, a member of the F-box protein family, plays a critical role in regulating protein degradation via the ubiquitin-proteasome system. It functions as a substrate-recognition component of E3 ubiquitin ligases, facilitating the ubiquitination of specific target proteins, which are subsequently marked for degradation. The dysregulation of FBXO4 has been implicated in various pathological conditions, including cancer, where it may influence cell cycle regulation and oncogenic signaling pathways. Recent studies suggest that FBXO4 may also participate in cellular processes such as apoptosis and response to oxidative stress. The production of recombinant FBXO4 protein allows researchers to investigate its structure-function relationship, elucidating its role in ubiquitination and potential therapeutic targets. Understanding FBXO4's interactions and mechanisms is vital for developing new strategies for cancer treatment and other diseases associated with protein misregulation. Thus, the recombinant expression and characterization of FBXO4 is not only important for basic research but also for potential clinical applications in designing therapeutics that could manipulate its activity or expression.
