Analytical Data
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Gene name
marA
- Application
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Alternative Names
marA；Macrophage-expressed gene 1 Protein
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0ACH5
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Expression Region
1-127aa
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AA Sequence
MSRRNTDAIT IHSILDWIED NLESPLSLEK VSERSGYSKW HLQRMFKKET GHSLGQYIRS RKMTEIAQKL KESNEPILYL AERYGFESQQ TLTRTFKNYF DVPPHKYRMT NMQGESRFLH PLNHYNS
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Molecular Weight
15.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MarA is a crucial regulatory protein in Escherichia coli that plays a significant role in multidrug resistance (MDR) by regulating the expression of various genes associated with efflux pumps and other stress response mechanisms. Its name arises from its role in modulating the expression of the mar (multidrug resistance) operon, which enables bacteria to survive in the presence of toxic compounds and antibiotics. The study of MarA is particularly important in the context of increasing antibiotic resistance, which poses a major challenge in clinical settings. MarA, as an AraC-type transcriptional regulator, can activate or repress the expression of genes that encode efflux pumps like AcrAB-TolC, which actively extrude a wide range of antibiotics from the bacterial cell, thereby reducing drug efficacy. Understanding the structure, function, and regulation of MarA provides valuable insights into the mechanisms of drug resistance and may lead to new strategies for combating bacterial infections. Research involving the recombination and characterization of MarA proteins elucidates their interaction with other regulatory networks and highlights potential targets for novel antimicrobial therapies. By studying the structural variations and functional dynamics of MarA, scientists aim to illuminate the pathways that lead to resistance, ultimately informing the development of compounds that can inhibit MarA activity or augment antibiotic effectiveness in resistant strains.
