Analytical Data
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基因名
Sap130
- Application
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别名
Sap130；Histone deacetylase complex subunit SAP130
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8BIH0
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表达区间
845-1057aa
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氨基酸序列
PRKQQHVISTEEGDMMETNSTDDEKSAAKSLLVKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLLRHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTNLEHDVYERLTNLQEGIIPKKKAATDDDLHRINELIQGNMQRCKLVMDQISEARDSMLKVLDHKDRVLKLLNKNGTVKKVSKLKRKEKV
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分子量
26.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sap130 is a crucial component of the spliceosome, a complex molecular machine responsible for the removal of introns from pre-mRNA in eukaryotic cells. As a part of the SR protein family, Sap130 plays an integral role in regulating alternative splicing, which is vital for generating protein diversity and influencing various cellular processes. Research into the recombinant expression of Sap130 has gained momentum due to its implications in understanding splicing mechanisms and its potential links to various diseases, including cancer and genetic disorders. By producing recombinant Sap130, scientists aim to investigate its structural properties and interactions with other spliceosomal components, which could reveal insights into the intricacies of RNA processing. Moreover, understanding the functional roles of Sap130 at a molecular level can elucidate how disruptions in splicing contribute to disease pathology, thereby offering potential avenues for therapeutic intervention. Given the increasing recognition of alternative splicing as a key regulatory mechanism in gene expression, studies on Sap130 and its functionalities are poised to significantly advance our knowledge in molecular biology, genetics, and disease mechanisms.
