Analytical Data
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基因名
GOLT1B
- Application
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别名
GOLT1B; GCT2; GOT1A; CGI-141; HDCMA39P; UNQ432/PRO793; Vesicle transport protein GOT1B; Germ cell tumor 2; Golgi transport 1 homolog B; Putative NF-kappa-B-activating protein 470; hGOT1a
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y3E0
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表达区间
1-138aa
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氨基酸序列
MISLTDTQKIGMGLTGFGVFFLFFGMILFFDKALLAIGNVLFVAGLAFVIGLERTFRFFFQKHKMKATGFFLGGVFVVLIGWPLIGMIFEIYGFFLLFRGFFPVVVGFIRRVPVLGSLLNLPGIRSFVDKVGESNNMV
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分子量
40.92 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GOLT1B (Golgi Transport 1B) is a protein that plays a critical role in the intracellular transport and processing of glycoproteins within the Golgi apparatus, a key organelle in the secretory pathway. Understanding the function and mechanism of GOLT1B is essential as it is implicated in various cellular processes, including protein sorting, glycosylation, and overall secretory pathway efficiency. Recent research has highlighted its potential links to diseases such as cancer and neurodegenerative disorders, where disruptions in Golgi function can lead to aberrant protein processing and homeostasis. The recombinant expression of GOLT1B allows researchers to explore its structural and functional properties in detail, fostering insights into its role in cellular biology. By investigating the biochemical characteristics and interaction networks of GOLT1B, scientists aim to elucidate its precise contribution to Golgi dynamics and its potential as a therapeutic target. This background underscores the significance of GOLT1B in both basic biological research and its implications for understanding disease mechanisms, highlighting the importance of developing recombinant versions of this protein for further study.
