Cat: PA2000-3828

Recombinant Human EBNA2 Protein,His

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Analytical Data

  • Gene name

    EBNA2

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    EBNA2;FOE;KIAA0261;WAPAL;Wings apart-like Protein homolog

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q69022

  • Expression Region

    247-454aa

  • AA Sequence

    MPTYYLALHGGQSYNLIVDTDMSGNPSLSVIPTNPYQEQLSNNPLIQLQI VVGENTGAPAPPQPPPPPPPPPPPERRDAWTQEPLPLDMNPLGSDASQGP LASSIRMLCMAQYLLRNARGQQGLLRPLGPQTRSQVTLERQPVHNPRQEA PIILLQSPAPPRFTPVPMVALGHTLQPTPPPRPTLPQPRIPLIIPPRHTN QPATTPPTAPQRLTLGHQLSLPPHPPPHQSTPHCSSDSTGLPPPPTSYSI PSMTLSPEPLPPPAAPAHPLPGVIYDQQALPPTPGPPWWPPVRDPTPTTQ TPPTNTKQGPDQGQGRGRWRGRGRSKGRGRMHKLPEPRRPGPDTSSPSMP QLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDS EEPPFLFPSDWYPPTLEPAELDESWEGIFETTESHSSDEENVGGPSKRPR TSTQ

  • Molecular Weight

    48.9 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

EBNA2 (Epstein-Barr virus nuclear antigen 2) is a crucial oncogenic protein encoded by the Epstein-Barr virus (EBV), which is associated with various lymphoproliferative diseases, including Hodgkin lymphoma and Burkitt lymphoma. Understanding EBNA2's function is essential due to its role in cell transformation and regulation of viral gene expression, particularly in B cells. EBNA2 acts by binding to specific enhancer regions of the viral genome, promoting transcriptional activation of other viral genes and driving host cellular pathways that lead to uncontrolled cell proliferation. Research has focused on the structural and functional characterization of EBNA2, revealing its intricate interactions with cellular proteins and the transcriptional machinery. These studies aim to elucidate the molecular mechanisms by which EBNA2 exerts its oncogenic effects, providing insights into potential therapeutic targets for EBV-associated malignancies. Furthermore, the recombination of EBNA2 protein for experimental purposes has enabled researchers to develop targeted therapies and diagnostic tools, fostering a deeper understanding of EBV pathogenesis and its implications in cancer biology.

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