Analytical Data
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Gene name
EBNA2
- Application
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Alternative Names
EBNA2;FOE;KIAA0261;WAPAL;Wings apart-like Protein homolog
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q69022
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Expression Region
247-454aa
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AA Sequence
MPTYYLALHGGQSYNLIVDTDMSGNPSLSVIPTNPYQEQLSNNPLIQLQI VVGENTGAPAPPQPPPPPPPPPPPERRDAWTQEPLPLDMNPLGSDASQGP LASSIRMLCMAQYLLRNARGQQGLLRPLGPQTRSQVTLERQPVHNPRQEA PIILLQSPAPPRFTPVPMVALGHTLQPTPPPRPTLPQPRIPLIIPPRHTN QPATTPPTAPQRLTLGHQLSLPPHPPPHQSTPHCSSDSTGLPPPPTSYSI PSMTLSPEPLPPPAAPAHPLPGVIYDQQALPPTPGPPWWPPVRDPTPTTQ TPPTNTKQGPDQGQGRGRWRGRGRSKGRGRMHKLPEPRRPGPDTSSPSMP QLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDS EEPPFLFPSDWYPPTLEPAELDESWEGIFETTESHSSDEENVGGPSKRPR TSTQ
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Molecular Weight
48.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EBNA2 (Epstein-Barr virus nuclear antigen 2) is a crucial oncogenic protein encoded by the Epstein-Barr virus (EBV), which is associated with various lymphoproliferative diseases, including Hodgkin lymphoma and Burkitt lymphoma. Understanding EBNA2's function is essential due to its role in cell transformation and regulation of viral gene expression, particularly in B cells. EBNA2 acts by binding to specific enhancer regions of the viral genome, promoting transcriptional activation of other viral genes and driving host cellular pathways that lead to uncontrolled cell proliferation. Research has focused on the structural and functional characterization of EBNA2, revealing its intricate interactions with cellular proteins and the transcriptional machinery. These studies aim to elucidate the molecular mechanisms by which EBNA2 exerts its oncogenic effects, providing insights into potential therapeutic targets for EBV-associated malignancies. Furthermore, the recombination of EBNA2 protein for experimental purposes has enabled researchers to develop targeted therapies and diagnostic tools, fostering a deeper understanding of EBV pathogenesis and its implications in cancer biology.











