Cat: PA1000-9645

Recombinant Human KDM4C Protein,His

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Analytical Data

  • Gene name

    KDM4C

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    KDM4C;GASC1;JHDM3C;JMJD2C;Lysine-specific demethylase 4C

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9H3R0

  • Expression Region

    1-460aa

  • AA Sequence

    MEVAEVESPL NPSCKIMTFR PSMEEFREFN KYLAYMESKG AHRAGLAKVI PPKEWKPRQC YDDIDNLLIP APIQQMVTGQ SGLFTQYNIQ KKAMTVKEFR QLANSGKYCT PRYLDYEDLE RKYWKNLTFV APIYGADING SIYDEGVDEW NIARLNTVLD VVEEECGISI EGVNTPYLYF GMWKTTFAWH TEDMDLYSIN YLHFGEPKSW YAIPPEHGKR LERLAQGFFP SSSQGCDAFL RHKMTLISPS VLKKYGIPFD KITQEAGEFM ITFPYGYHAG FNHGFNCAES TNFATVRWID YGKVAKLCTC RKDMVKISMD IFVRKFQPDR YQLWKQGKDI YTIDHTKPTP ASTPEVKAWL QRRRKVRKAS RSFQCARSTS KRPKADEEEE VSDEVDGAEV PNPDSVTDDL KVSEKSEAAV KLRNTEASSE EESSASRMQV EQNLSDHIKL SGNSCLSTSV

  • Molecular Weight

    82 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

KDM4C, a member of the Jumonji domain-containing (JmjC) family of proteins, is a histone demethylase that specifically targets trimethylated lysine 9 and lysine 36 on histone H3. It plays a crucial role in regulating gene expression, cellular differentiation, and epigenetic modifications. Dysregulation of KDM4C has been implicated in various cancers, making it a significant target for therapeutic interventions. Research has shown that KDM4C's enzymatic activity influences not only histone methylation states but also the recruitment of transcriptional co-factors, thereby impacting chromatin dynamics and transcriptional regulation. Moreover, its overexpression in certain malignancies has been associated with poor prognosis, highlighting the need for a deeper understanding of its function and regulatory mechanisms. The exploration of KDM4C's structure and activity can provide insights into novel ways to manipulate epigenetic landscapes, potentially leading to innovative cancer therapies. Recent studies employing biochemical assays and structural biology approaches have unveiled the protein's interaction with various substrates, paving the way for targeted drug design aimed at modulating its activity in pathological contexts. This research not only enhances our understanding of KDM4C's biological significance but also opens up new avenues for therapeutic strategies that could leverage histone demethylases in cancer treatment.

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