Analytical Data
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基因名
pntA
- Application
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别名
pntA；NAD(P) transhydrogenase. mitochondrial
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P07001
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表达区间
1-510aa
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氨基酸序列
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
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分子量
54.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the pntA gene and its corresponding recombinant protein has gained substantial attention due to its crucial role in various biological processes, particularly in the context of microbial metabolism and energy production. Found in a range of bacteria, pntA encodes for a protein involved in the pyridine nucleotide transhydrogenase complex, which facilitates the interconversion of NADH and NADPH, essential cofactors in cellular redox reactions. Understanding the function and dynamics of the pntA protein is pivotal as it holds potential implications for biotechnological applications, such as biofuel production and bioremediation. Furthermore, insights into the pntA gene's regulation and expression can illuminate how bacteria adapt to different environmental conditions, offering strategies for manipulating microbial pathways for enhanced metabolic efficiency. Recent advances in recombinant DNA technology have enabled the production of pntA proteins in heterologous systems, allowing for detailed biochemical characterization and kinetic studies. This research not only contributes to the foundational understanding of bacterial energetics but also paves the way for innovative approaches in synthetic biology, where engineered microorganisms can be optimized for industrial processes. The multifaceted significance of pntA recombinant proteins thus underscores their importance in both basic research and applied sciences, making them a valuable target for further investigation.
