Analytical Data
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Gene name
ESAT6
- Application
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Alternative Names
ESAT6；esaT6;6 kDa early secretory antigenic target
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P9WNK7
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Expression Region
6-95aa
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AA Sequence
WNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA
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Molecular Weight
11.3kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ESAT-6 (Early Secreted Antigenic Target 6) is a 6-kDa protein produced by pathogenic strains of Mycobacterium tuberculosis, the causative agent of tuberculosis (TB). Its significance lies in its key role in the immune response, as it is recognized by T cells in infected individuals, making it a potential biomarker for TB diagnosis. Research into ESAT-6 has gained momentum due to its promising application in the development of vaccines and diagnostic tools. The protein is part of a family of virulence factors secreted by the Type VII secretion system, which plays a critical role in mycobacterial pathogenesis. Understanding the structure and function of ESAT-6 and its interactions with host immune cells is crucial for unraveling the mechanisms of TB infection and disease progression. Moreover, recombinant ESAT-6 proteins are being studied for their potential use in immunological assays, such as the QuantiFERON-TB Gold test, which helps differentiate between active and latent TB infections. The ongoing research on ESAT-6 encompasses various fields, including immunology, molecular biology, and vaccine development, aiming to enhance TB control strategies and improve global health outcomes. As TB remains a major public health challenge worldwide, the exploration of ESAT-6 as a recombinant protein provides a promising avenue for innovative approaches to diagnosis, treatment, and prevention.
