Analytical Data
-
Gene name
EIF4E
- Application
-
Alternative Names
EIF4E;Eukaryotic translation initiation factor 4E-binding Protein 2
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P06730
-
Expression Region
2-217aa
-
AA Sequence
ATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV
-
Molecular Weight
26.5 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Eukaryotic translation initiation factor 4E (eIF4E) is a crucial protein involved in the regulation of mRNA translation and plays a significant role in cellular processes such as growth, proliferation, and differentiation. It specifically binds to the 5' cap structure of mRNA, enabling the recruitment of other components of the translation initiation complex. Dysregulation of eIF4E has been implicated in various diseases, particularly cancer, where its overexpression can lead to enhanced translation of oncoproteins and the suppression of tumor suppressor genes. Research on recombinant eIF4E has gained momentum as scientists aim to elucidate its functional mechanisms, regulatory pathways, and interactions with other translation factors. The production of recombinant eIF4E allows for detailed studies of its structural properties, post-translational modifications, and the impact of specific mutations. Additionally, understanding how eIF4E activity is controlled can provide insights into therapeutic strategies for diseases linked to its dysregulation. Given its pivotal role in translation and cell growth, eIF4E remains a focal point in molecular biology and oncology research, making it a promising target for the development of novel interventions that could inhibit its function or rectify its aberrant signaling in pathological conditions.
