Analytical Data
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基因名
GNE
- Application
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别名
UDP-GlcNAc-2-epimerase/ManAc kinase;hydrolyzing;UDP-GlcNAc-2-epimerase;Uridine diphosphate-N-acetylglucosamine-2-epimerase;ManAc kinase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y223
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表达区间
1-722aa
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氨基酸序列
MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY
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分子量
86.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GNE (glucosamine-6-phosphate N-acetyltransferase) is an essential enzyme involved in the biosynthetic pathway of sialic acids, which are crucial for various biological processes, including cell signaling and immune response. Mutations in the GNE gene are associated with a rare genetic disorder known as GNE myopathy, characterized by progressive muscle weakness and atrophy. Understanding the structure and function of GNE, along with the mechanisms by which mutations disrupt its activity, is critical for developing potential therapeutic strategies. Researchers have increasingly turned to recombinant protein techniques to produce GNE for biochemical and biophysical studies, allowing for detailed investigation of its enzymatic activity, interaction with substrates, and the effects of pathogenic mutations. By characterizing the recombinant GNE protein, scientists aim to elucidate the molecular basis of GNE myopathy, paving the way for targeted interventions and improved management of the disease. Additionally, studying the recombinant protein can provide insights into the regulatory mechanisms governing sialic acid biosynthesis, which may have broader implications in understanding other metabolic disorders where sialic acids play a significant role. The ongoing research in this field emphasizes the importance of GNE not only in the context of muscle diseases but also in the broader landscape of glycobiology and its impact on human health.
