Analytical Data
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Gene name
EIF3I
- Application
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Alternative Names
EIF3I；EIF3S2；TRIP1;Eukaryotic translation initiation factor 3 subunit I
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13347
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Expression Region
1-325aa
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AA Sequence
MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA
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Molecular Weight
39 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF3I, or Eukaryotic Translation Initiation Factor 3 Subunit I, plays a critical role in the initiation of protein synthesis, functioning as a component of the eukaryotic translation initiation factor 3 (eIF3) complex. This complex is essential for bridging the small ribosomal subunit and the mRNA, facilitating the assembly of the translation machinery at the start codon. Research has shown that EIF3I is not only vital for normal cell growth and development but also implicated in various pathophysiological conditions, including cancer. Abnormal expression levels of EIF3I have been associated with tumor progression, suggesting that it may influence the translation of specific mRNAs involved in cell proliferation and survival. As a result, there is significant interest in studying EIF3I and its interactions within the translational machinery to better understand its role in cancer biology and to explore its potential as a therapeutic target. Advances in recombinant protein expression techniques have enabled the production of EIF3I for structural and functional analyses, facilitating the determination of its precise role in translation initiation and its interactions with other eIF3 subunits and factors. This research could pave the way for novel strategies in cancer treatment and provide insights into the regulation of gene expression at the translational level.
