Analytical Data
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Gene name
HS6ST1
- Application
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Alternative Names
HS6ST1；HS6ST；Heparan-sulfate 6-O-sulfotransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60243
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Expression Region
38-411aa
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AA Sequence
PGLSLGAPGGRAPPDDLDLFPTPDPHYEKKYYFPVRELERSLRFDMKGDDVIVFLHIQKTGGTTFGRHLVQNVRLEVPCDCRPGQKKCTCYRPNRRETWLFSRFSTGWSCGLHADWTELTNCVPGVLDRRDSAALRTPRKFYYITLLRDPVSRYLSEWRHVQRGATWKTSLHMCDGRTPTPEELPPCYEGTDWSGCTLQEFMDCPYNLANNRQVRMLADLSLVGCYNLSFIPEGKRAQLLLESAKKNLRGMAFFGLTEFQRKTQYLFERTFNLKFIRPFMQYNSTRAGGVEVDEDTIRRIEELNDLDMQLYDYAKDLFQQRYQYKRQLERREQRLRSREERLLHRAKEALPREDADEPGRVPTEDYMSHIIEKW
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Molecular Weight
51.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HS6ST1, or heparan sulfate 6-O-sulfotransferase 1, is an essential enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that plays critical roles in various physiological processes, including cell signaling, proliferation, and differentiation. The importance of HS6ST1 has gained attention due to its regulatory function in modulating the activity of growth factors and chemokines through the sulfation of specific heparan sulfate chains. This modification can influence various biological pathways, including tumor progression and development, making HS6ST1 a potential target for therapeutic interventions in cancers and other diseases associated with aberrant heparan sulfate biosynthesis. Recent studies have demonstrated that HS6ST1 is not only crucial for normal cellular functions but also implicated in pathological conditions, highlighting the need for characterization of its structure and function. Research into recombinant HS6ST1 protein has become particularly important for elucidating its enzymatic mechanisms and interactions with various substrates, which can pave the way for the development of selective inhibitors or modulators. Understanding the role of HS6ST1 at a molecular level could provide deeper insights into its involvement in disease mechanisms and open new avenues for targeted therapies, thus establishing its significance in the field of glycobiology and medicinal chemistry. Therefore, the production and purification of recombinant HS6ST1 protein serve as foundational steps in exploring its functional implications and therapeutic potential.
