Analytical Data
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基因名
PLD2
- Application
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别名
PLD2；Phospholipase D2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14939
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表达区间
1-933aa
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氨基酸序列
MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQ SLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTT KKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRA GPEGSTRHAASKQKYLENYLNCLLTMSFYRNYHAMTEFLEVSQLSFIPDL GRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCL ETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQAR WWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVAD AILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSIL LFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQ VVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLS HNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGL PARDLARHFIQRWNFTKTTKAKYKTPIYPYLLPKSTSTANQLPFTLPGGQ CTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDG RTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQ AILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVS ELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSL MNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMA ESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQ GHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT
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分子量
105.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLD2 (Phospholipase D2) is a key enzyme involved in the hydrolysis of phosphatidylcholine, producing phosphatidic acid, which acts as an important signaling molecule in various cellular processes. Its role has gained attention due to its involvement in critical biological functions such as cell proliferation, differentiation, and migration, as well as in the regulation of membrane trafficking. Dysregulation of PLD2 has been linked to several pathological conditions, including cancer, cardiovascular diseases, and neurological disorders. Research has focused on understanding the molecular mechanisms by which PLD2 influences cellular signaling pathways and how it interacts with other proteins and lipids in the cellular environment. The production of recombinant PLD2 protein has become an essential tool for exploring its enzymatic properties, structural biology, and potential therapeutic applications. By utilizing techniques like protein purification and characterization, scientists aim to elucidate the functional roles of PLD2 in various cellular contexts, thereby aiding in the development of targeted interventions for diseases associated with PLD2 dysregulation.
