Analytical Data
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Gene name
PLD
- Application
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Alternative Names
PLD；Phospholipase D1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N2A8
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Expression Region
1-252aa
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AA Sequence
MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT
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Molecular Weight
28.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Phospholipase D (PLD) is a crucial enzyme involved in various cellular processes, including membrane remodeling, signal transduction, and lipid metabolism. Its primary function is to catalyze the hydrolysis of phosphatidylcholine to produce phosphatidic acid and choline, playing a pivotal role in the regulation of cell growth, survival, and motility. Research on PLD has gained momentum due to its implications in diverse biological processes and its potential links to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. Increased understanding of PLD and its mechanisms can reveal novel therapeutic targets and strategies for treating these diseases. Moreover, the production of recombinant PLD proteins has become essential for detailed structural and functional studies, enabling researchers to elucidate the enzyme's role in specific cellular contexts. By utilizing advanced recombinant DNA technology and expression systems, scientists can generate pure PLD proteins for biochemical assays, drug screening, and cellular studies, ultimately contributing to the development of PLD-targeted therapies. This ongoing research not only enhances our understanding of PLD's biology but also underscores its significance in pharmaceutical applications, making it a focal point of interest in contemporary biomedical research.
