Analytical Data
-
Gene name
DNMT3L
- Application
-
Alternative Names
DNMT3L;DNA (cytosine-5)-methyltransferase 3-like
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UJW3
-
Expression Region
1-386aa
-
AA Sequence
MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIE DICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICC SGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSR SGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIK KELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGH TCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASR FLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNK QSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL
-
Molecular Weight
70 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNMT3L (DNA methyltransferase 3-like protein) is a crucial regulator in the process of DNA methylation, which plays a significant role in epigenetic modifications that influence gene expression and developmental processes. Unlike other DNA methyltransferases, DNMT3L lacks catalytic activity but serves as an essential cofactor for DNMT3A and DNMT3B, promoting their activity in de novo methylation. The study of DNMT3L has gained prominence due to its involvement in gametogenesis, embryonic development, and potential implications in diseases such as cancer and genetic disorders. Research has revealed that DNMT3L is vital for normal spermatogenesis and oogenesis, and its aberrant expression has been linked to infertility and reproductive issues. Moreover, understanding the molecular mechanisms by which DNMT3L interacts with other proteins and DNA is critical for elucidating its role in establishing and maintaining methylation patterns. Recent advances in recombinant protein technology have enabled the production and characterization of DNMT3L, facilitating investigations into its structure-function relationships and biochemical properties. Overall, the exploration of DNMT3L not only enhances our comprehension of epigenetic regulation but also opens avenues for therapeutic strategies targeting epigenetic modifications in various diseases.
