Analytical Data
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基因名
DNMT3L
- Application
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别名
DNMT3L;DNA (cytosine-5)-methyltransferase 3-like
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UJW3
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表达区间
1-386aa
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氨基酸序列
MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIE DICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICC SGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSR SGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIK KELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGH TCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASR FLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNK QSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL
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分子量
70 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNMT3L (DNA methyltransferase 3-like protein) is a crucial regulator in the process of DNA methylation, which plays a significant role in epigenetic modifications that influence gene expression and developmental processes. Unlike other DNA methyltransferases, DNMT3L lacks catalytic activity but serves as an essential cofactor for DNMT3A and DNMT3B, promoting their activity in de novo methylation. The study of DNMT3L has gained prominence due to its involvement in gametogenesis, embryonic development, and potential implications in diseases such as cancer and genetic disorders. Research has revealed that DNMT3L is vital for normal spermatogenesis and oogenesis, and its aberrant expression has been linked to infertility and reproductive issues. Moreover, understanding the molecular mechanisms by which DNMT3L interacts with other proteins and DNA is critical for elucidating its role in establishing and maintaining methylation patterns. Recent advances in recombinant protein technology have enabled the production and characterization of DNMT3L, facilitating investigations into its structure-function relationships and biochemical properties. Overall, the exploration of DNMT3L not only enhances our comprehension of epigenetic regulation but also opens avenues for therapeutic strategies targeting epigenetic modifications in various diseases.
