Cat: PA1000-9533

Recombinant Human TOR3A Protein,His

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Analytical Data

  • Gene name

    TOR3A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    TOR3A;ADIR;Torsin-3A

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9H497

  • Expression Region

    26-397aa

  • AA Sequence

    ASRPW EGTDEPGSAW AWPGFQRLQE QLRAAGALSK RYWTLFSCQV WPDDCDEDEE AATGPLGWRL PLLGQRYLDL LTTWYCSFKD CCPRGDCRIS NNFTGLEWDL NVRLHGQHLV QQLVLRTVRG YLETPQPEKA LALSFHGWSG TGKNFVARML VENLYRDGLM SDCVRMFIAT FHFPHPKYVD LYKEQLMSQI RETQQLCHQT LFIFDEAEKL HPGLLEVLGP HLERRAPEGH RAESPWTIFL FLSNLRGDII NEVVLKLLKA GWSREEITME HLEPHLQAEI VETIDNGFGH SRLVKENLID YFIPFLPLEY RHVRLCARDA FLSQELLYKE ETLDEIAQMM VYVPKEEQLF SSQGCKSISQ RINYFLS

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

TOR3A (Target of Rapamycin Complex 3A) is a crucial protein complex involved in regulating various cellular processes, including cell growth, proliferation, and metabolism. It has garnered significant attention in the field of molecular biology and cancer research due to its role in the phosphoinositide 3-kinase (PI3K) signaling pathway, which is frequently dysregulated in various cancers. Understanding the structure and function of TOR3A is essential for deciphering its interactions with other cellular components and pathways. The reconstitution of TOR3A as a recombinant protein provides a valuable model for studying its biochemical properties and functional mechanisms. Researchers aim to explore how TOR3A contributes to tumorigenesis, its potential as a therapeutic target, and its involvement in resistance mechanisms to existing therapies. The recombinant expression system allows for the production of purified TOR3A, facilitating detailed studies of its activity, interaction with drugs, and response to cellular stress. This research is pivotal for developing new cancer treatments and enhancing our understanding of cellular growth regulation. Overall, the investigation of TOR3A and its signaling pathways holds promise for unveiling novel therapeutic strategies and improving patient outcomes in cancer therapy.

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