Analytical Data
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Gene name
RPL3
- Application
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Alternative Names
RPL3；Large ribosomal subunit Protein uL3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P39023
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Expression Region
2-247aa
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AA Sequence
SHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRG
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Molecular Weight
55.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RPL3 is a crucial component of the ribosomal protein family, playing a significant role in ribosome biogenesis and protein synthesis. It is a vital part of the large ribosomal subunit and is involved in the assembly and stabilization of ribosomal RNA (rRNA) and proteins, contributing to ribosome function and cellular homeostasis. Studies have suggested that RPL3 is not only essential for basic cellular processes but also plays a role in various diseases, including cancer, where its dysregulation may affect protein synthesis pathways. Given its involvement in cellular metabolism and gene regulation, researchers are increasingly focusing on recombinant RPL3 protein to elucidate its structure-function relationships and interactions with other ribosomal components. By producing and purifying recombinant RPL3, scientists aim to explore its biochemical properties and potential molecular mechanisms by which it influences ribosomal assembly and translation processes. Understanding RPL3’s functional dynamics may provide insights into therapeutic strategies for diseases associated with ribosomal dysfunction and enhance our comprehension of the fundamental processes of cellular biology.
