Analytical Data
-
Gene name
CYC1
- Application
-
Alternative Names
CYC1；Cytochrome c1. heme Protein. mitochondrial
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P08574
-
Expression Region
85-325aa
-
AA Sequence
SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCASCHSMDFVAYRHLVGVCYTEDEAKELAAEVEVQDGPNEDGEMFMRPGKLFDYFPKPYPNSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYRPPK
-
Molecular Weight
54.4 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYC1, or cytochrome c1, is a crucial component of the mitochondrial respiratory chain, playing a vital role in cellular energy production through its involvement in electron transport and ATP synthesis. Research on CYC1 recombinant protein has gained significant attention due to its potential applications in bioenergetics and therapeutic interventions for mitochondrial diseases. The recombinant expression of CYC1 allows for detailed studies of its biochemical properties, interactions with other mitochondrial proteins, and its role in the oxidative phosphorylation pathway. Furthermore, understanding the structure-function relationship of CYC1 can provide insights into the mechanisms of various mitochondrial dysfunctions that lead to metabolic disorders. Advances in biotechnology, such as the use of yeast and bacterial systems for protein expression, have facilitated the purification of CYC1, enabling researchers to investigate its kinetic properties and explore potential avenues for drug development. Given the increasing prevalence of mitochondrial diseases, the study of CYC1 and its recombinant form is crucial for developing diagnostic and therapeutic strategies aimed at improving mitochondrial function and restoring energy metabolism in affected individuals.
