Analytical Data
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基因名
CUL1
- Application
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别名
CUL1;Cullin-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13616
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表达区间
1-776aa
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氨基酸序列
MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVY NYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYL TNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECD EGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRL ISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTE FLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHL EIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLA AIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACG RFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVM VVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEY TSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQS CTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYT LQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLV LEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTH KNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIK KCIDILIEKEYLERVDGEKDTYSYLA
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分子量
116 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CUL1, or Cullin-1, is a pivotal protein that plays a crucial role in the ubiquitin-proteasome system, a key regulatory pathway responsible for protein degradation and turnover within cells. As a member of the Cullin family of proteins, CUL1 serves as a scaffold that assembles E3 ubiquitin ligase complexes, facilitating the transfer of ubiquitin molecules to target proteins. This modification often signals for protein degradation, influencing various cellular processes such as cell cycle regulation, signal transduction, and responses to stress. Dysregulation of CUL1 and its related pathways has been implicated in several diseases, including cancer, where aberrant protein degradation can lead to uncontrolled cell proliferation and survival. The study of CUL1 recombinant proteins is vital for elucidating its functional mechanisms and interactions within ubiquitin ligase complexes. Additionally, exploring the structural and functional properties of CUL1 can provide insights into how it regulates substrate specificity and activity, which is essential for understanding its role in pathological conditions. Research into CUL1 not only enhances our comprehension of fundamental biological mechanisms but also holds potential for the development of targeted therapeutic strategies aimed at modulating its activity in disease contexts. By harnessing recombinant protein technology, scientists can investigate CUL1 in detail, paving the way for discovering novel methods to manipulate its function for therapeutic benefits.
