Analytical Data
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Gene name
CSTF1
- Application
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Alternative Names
CSTF1;Cleavage stimulation factor subunit 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q05048
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Expression Region
1-431aa
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AA Sequence
MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DVEHHHHHH
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Molecular Weight
49 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CSTF1, or Cleavage Stimulating Factor 1, is an essential component of the cleavage and polyadenylation machinery that plays a crucial role in the post-transcriptional regulation of gene expression. It is involved in the processing of pre-mRNA, facilitating the cleavage of nascent transcripts and the subsequent addition of poly(A) tails, which are vital for mRNA stability, export, and translation. Research on CSTF1 has garnered significant interest due to its implications in various biological processes, including cell proliferation, differentiation, and response to stress. Furthermore, abnormalities in CSTF1 expression or function have been linked to several diseases, including cancer and neurodegenerative disorders. Understanding the structure, function, and regulatory mechanisms of CSTF1 is therefore critical for deciphering its role in normal physiology and disease states. Recent studies utilizing recombinant CSTF1 proteins have enabled the exploration of its biochemical properties, interaction with other nuclear factors, and the impact of post-translational modifications. These investigations are paving the way for potential therapeutic interventions targeting CSTF1-related pathways, highlighting the protein's significance in cellular homeostasis and disease etiology.
