Analytical Data
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Gene name
COA4
- Application
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Alternative Names
COA4；CHCHD8；E2IG2;Cytochrome c oxidase assembly factor 4 homolog. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NYJ1
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Expression Region
1-87aa
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AA Sequence
MSTSVPQGHT WTQRVKKDDE EEDPLDQLIS RSGCAASHFA VQECMAQHQD WRQCQPQVQA FKDCMSEQQA RRQEELQRRQ EQAGAHH
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
COA4 (Coenzyme A Synthetase 4) is a non-canonical enzyme that plays a critical role in the mitochondrial bioenergetics, particularly in the biosynthesis of Coenzyme A (CoA), an essential cofactor involved in various metabolic pathways. Research on COA4 has gained traction due to its potential implications in mitochondrial dysfunction and related diseases, including metabolic disorders and neurodegenerative conditions. As mitochondrial health is closely linked to overall cellular energy metabolism and oxidative stress regulation, understanding the structure and function of COA4 is paramount. Recent studies have focused on the recombinant expression and characterization of COA4 protein to elucidate its biochemical properties and regulatory mechanisms. This research aims to identify the enzymatic activity of COA4, its interaction with other metabolic partners, and its role in cellular energy homeostasis. By generating recombinant COA4, researchers can explore various factors affecting its activity and stability, which may yield novel insights into therapeutic targets for diseases associated with mitochondrial dysfunction. Such investigations not only enhance our fundamental understanding of CoA metabolism but also pave the way for the development of targeted interventions in related health conditions.
