Analytical Data
-
基因名
ENGASE
- Application
-
别名
Cytosolic endo-beta-N-acetylglucosaminidase; DKFZp434P174; ENASE_HUMAN; ENGase; FLJ21865; Mannosyl glycoprotein endo beta N acetylglucosaminidase
-
种属
Human
-
表达系统
E. coli
-
标签
N- His-SUMO
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q8NFI3
-
表达区间
1-377aa
-
分子量
59.1 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ENGASE, or endo-β-N-acetylglucosaminidase, is an enzyme that plays a crucial role in the deglycosylation of glycoproteins, contributing to various biological processes and cellular functions. The study of ENGASE is significant due to its potential applications in both fundamental research and biotechnology. Understanding the mechanisms by which ENGASE operates can provide insights into protein folding, stability, and the regulation of glycoprotein turnover in the cell. Furthermore, ENGASE can be exploited in therapeutic contexts, such as developing strategies for treating diseases associated with glycoprotein misfolding or overproduction. Research on ENGASE involves a combination of structural biology, enzymology, and molecular genetics, aiming to elucidate the enzyme’s structure-function relationship and its interactions with substrates. Advances in recombinant protein technology enable the production of this enzyme in large quantities, allowing for detailed studies on its biochemical properties and applications in drug development and diagnostics. Overall, the exploration of ENGASE not only enhances our understanding of glycoprotein biology but also opens new avenues for innovative therapeutic interventions.
