Analytical Data
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基因名
ALKBH5
- Application
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别名
(Alkylated DNA repair protein alkB homolog 5)(Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5)
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种属
Human
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6P6C2
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表达区间
2-394aa
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分子量
48.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ALKBH5, an RNA demethylase belonging to the AlkB family of proteins, plays a crucial role in RNA metabolism by removing N6-methyladenosine (m6A) modifications from RNA molecules. This demethylation activity is essential for various cellular processes, including mRNA stability, splicing, translation, and the regulation of gene expression. Dysregulation of m6A modification and the function of ALKBH5 have been implicated in various diseases, including cancer, neurological disorders, and metabolic diseases. Studies have shown that ALKBH5 can modulate stem cell pluripotency, influence cellular responses to environmental stresses, and affect the fate of certain mRNAs, highlighting its significant role in post-transcriptional regulation. Moreover, the characterization of ALKBH5 as a potential biomarker or therapeutic target in cancer has opened up new avenues for research. As scientists continue to investigate the intricate mechanisms of m6A modification and the functional implications of ALKBH5, the development of recombinant ALKBH5 protein becomes essential for understanding its structure-function relationship, enzymatic activity, and potential interactions with other molecular partners. Such studies will not only enhance our comprehension of RNA methylation but may also contribute to the design of novel therapeutic strategies targeting m6A regulators in diseases driven by dysregulated RNA metabolism.
