Analytical Data
-
Gene name
DFFB
- Application
-
Alternative Names
(Caspase-activated deoxyribonuclease)(CAD)(Caspase-activated DNase)(Caspase-activated nuclease)(CPAN)(DNA fragmentation factor 40 kDa subunit)(DFF-40)
-
Species
Human
-
Source
E. coli
-
Tag
N- His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O76075
-
Expression Region
1-338aa
-
Molecular Weight
45.1 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DFFB, also known as DNA fragmentation factor subunit B, plays a crucial role in the apoptotic process, particularly in the execution phase of apoptosis, where it participates in the cleavage of chromosomal DNA. The study of DFFB has gained traction due to its involvement in various physiological and pathological processes, including cancer progression and neurodegenerative diseases. Research indicates that the improper regulation of DFFB can lead to abnormal apoptosis, which is linked to tumor development and resistance to therapy. Additionally, DFFB is a key component of the caspase-activated DNase (CAD) complex, a pivotal player in DNA fragmentation during cell death. Understanding the molecular mechanisms underlying DFFB function and its interactions with other apoptotic proteins could provide insights into therapeutic targets for diseases characterized by dysregulated apoptosis. Furthermore, the recombinant expression of DFFB has enabled scientists to investigate its biochemical properties and functional domains, thereby advancing the development of apoptosis-modulating drugs. As a result, the exploration of DFFB and its role in apoptosis not only contributes to the fundamental understanding of cell death mechanisms but also has significant implications for disease treatment strategies.
