Analytical Data
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Gene name
NES
- Application
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Alternative Names
NES；Nestin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48681
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Expression Region
1321-1569aa
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AA Sequence
PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPL
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Molecular Weight
27.8kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of NES (Nuclear Export Signal) recombinant proteins has gained considerable attention in the field of molecular biology due to their crucial role in the active transport of proteins from the nucleus to the cytoplasm. NESs are typically short peptide sequences within proteins that facilitate their binding to export receptors, such as CRM1 (Chromosomal Maintenance 1), which transport them across the nuclear pore complex. This process is essential for various cellular functions, including gene expression regulation, signal transduction, and the maintenance of cellular homeostasis. Dysregulation of nuclear export can lead to various diseases, including cancer and neurodegenerative disorders, highlighting the significance of understanding NESs. Recent advancements in protein engineering and expression systems allow researchers to produce recombinant NES proteins for structural and functional studies. These studies aim to elucidate the mechanisms of nuclear export, identify novel NESs, and explore their interactions with export receptors. Additionally, recombinant NES proteins have potential therapeutic applications, as they can be used to design targeted drug delivery systems or inhibit the export of oncogenic proteins. As research progresses, the insights gained from studying NES recombinant proteins could lead to innovative strategies for disease treatment and the development of biomolecular tools for various biotechnological applications.
