Analytical Data
-
Gene name
G75V,T76I,SYLTPGD247-253del,L452Q,F490S,D614G,T859N
- Application
-
Alternative Names
(S glycoprotein)(Peplomer protein)(Spike protein S1)(Spike protein S2)
-
Species
SARS-CoV-2
-
Source
HEK293
-
Tag
N- His & C- Flag
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P0DTC2
-
Expression Region
16-685aa(G75V,T76I,SYLTPGD247-253del,L452Q,F490S,D614G,T859N)
-
Molecular Weight
78.9 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of recombinant proteins such as G75V, T76I, SYLTPGD247-253del, L452Q, F490S, D614G, and T859N is crucial in understanding the evolution and adaptation of pathogenic viruses, particularly SARS-CoV-2, the virus responsible for COVID-19. Each of these mutations has been associated with enhanced transmissibility, immune escape, or altered virulence. For instance, the D614G mutation has shown increased infectivity and is prevalent in multiple variants of the virus. Similarly, the L452Q mutation has raised concerns due to its potential impact on vaccine efficacy and therapeutic neutralization. The deletion SYLTPGD247-253 is thought to influence the virus's ability to evade host immune responses. As the virus continues to mutate, studying these recombinant proteins helps researchers understand how changes in the spike protein can affect viral behavior and interaction with host cells. These insights are vital for the development of effective vaccines and therapeutics, as well as for informing public health strategies in response to emerging variants. Understanding these modifications at the molecular level is key to anticipating viral behavior and improving control measures against COVID-19 and similar infectious diseases in the future. Through detailed molecular characterization of these mutations, researchers aim to enhance our preparedness against possible future pandemics driven by viral mutations.
