Analytical Data
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Gene name
SIGLEC8
- Application
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Alternative Names
SIGLEC8；SAF2；Sialic acid-binding Ig-like lectin 8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NYZ4
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Expression Region
1-499aa
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AA Sequence
MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG
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Molecular Weight
54 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Siglec-8 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family, primarily expressed on immune cells, particularly eosinophils and mast cells. Its role has gained significant attention in recent years due to its involvement in regulating immune responses, particularly in allergic and autoimmune conditions. Siglec-8 functions by promoting cell apoptosis and inhibiting activation, which helps in controlling excessive inflammation. Researchers have been interested in developing recombinant forms of Siglec-8 to better understand its mechanisms and therapeutic potential. These recombinant proteins can be used to elucidate the signaling pathways involved in immune modulation and to explore potential applications in treating conditions such as asthma, atopic dermatitis, and other allergic diseases. By studying the structure and function of Siglec-8, scientists aim to design targeted therapies that harness its regulatory functions to suppress unwanted immune responses while preserving protective immunity. The ongoing research into Siglec-8 recombinant proteins promises to provide insights into innovative treatment strategies that could significantly impact the management of various immune-related disorders.
