Analytical Data
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基因名
CHST9
- Application
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别名
CHST9；Carbohydrate sulfotransferase 9
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7L1S5
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表达区间
1-443aa
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氨基酸序列
MQPSEMVMNPKQVFLSVLIFGVAGLLLFMYLQVWIEEQHTGRVEKRREQKVTSGWGPVKYLRPVPRIMSTEKIQEHITNQNPKFHMPEDVREKKENLLLNSERSTRLLTKTSHSQGGDQALSKSTGSPTEKLIEKRQGAKTVFNKFSNMNWPVDIHPLNKSLVKDNKWKKTEETQEKRRSFLQEFCKKYGGVSHHQSHLFHTVSRIYVEDKHKILYCEVPKAGCSNWKRILMVLNGLASSAYNISHNAVHYGKHLKKLDSFDLKGIYTRLNTYTKAVFVRDPMERLVSAFRDKFEHPNSYYHPVFGKAIIKKYRPNACEEALINGSGVKFKEFIHYLLDSHRPVGMDIHWEKVSKLCYPCLINYDFVGKFETLEEDANYFLQMIGAPKELKFPNFKDRHSSDERTNAQVVRQYLKDLTRTERQLIYDFYYLDYLMFNYTTPFL
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分子量
52 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHST9, or carbohydrate sulfotransferase 9, is an enzyme that plays a crucial role in the sulfation of carbohydrate moieties, particularly in glycosaminoglycans and proteoglycans. These sulfated carbohydrates are pivotal for various biological processes, including cell signaling, adhesion, and development. Dysregulation of sulfation patterns due to altered CHST9 activity has been implicated in several pathological conditions, including cancer and congenital disorders. Research into the recombinant expression of CHST9 protein is driven by the need to understand its functional mechanisms and to explore its potential as a therapeutic target. Producing recombinant CHST9 allows for detailed biochemical assays and structural studies, providing insights into its substrate specificity and interaction with co-factors. Furthermore, understanding CHST9's role in cellular processes can pave the way for novel strategies in drug development. Recent advancements in protein engineering and expression systems, such as using mammalian cell lines or yeast, have facilitated the generation of active CHST9 proteins, enabling researchers to investigate its biological functions and the effects of sulfation. As the role of carbohydrate sulfation becomes increasingly recognized in various diseases, CHST9 research is poised to contribute significantly to the fields of glycobiology and therapeutic innovation.
